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and *HaeIII* or *RsaI* endonucleases. DNA sequencing was carried out by Beijing Biomed Biotech Company (Beijing, China).
Sequencing of the *E. coli* MFS protein {#Sec18}
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Because we found a single base insertion in the protein sequence derived from the *E. coli* K-12 strain (ATCC 29381), we retrieved the genome sequence from GenBank (NC_ 50e0806aeb gragra
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